Render Target: STATIC
Render Timestamp: 2024-12-02T11:52:52.736Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:58:30.023
Product last modified at: 2024-10-17T22:30:09.381Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GUCY2C (E6I2U) Rabbit mAb #37517

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 145, 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #92931.

    Protocol

    Specificity / Sensitivity

    GUCY2C (E6I2U) Rabbit mAb recognizes endogenous levels of total GUCY2C protein. Non-specific staining was observed in acinar cells of the salivary gland and keratinized epithelium of the skin by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys1046 of human GUCY2C protein.

    Background

    Guanylyl cyclase C (GUCY2C) is a type I transmembrane glycoprotein whose expression in normal tissue is largely restricted to the apical membranes of polarized epithelial cells of the intestine (1,2). The guanylyl cyclase activity within the intracellular domain of GUCY2C is activated by binding exogenous bacterial enterotoxins and the endogenous peptide hormone agonists, guanylin and uroguanylin, which facilitates production of the second messenger cGMP (3-5). GUCY2C signaling plays a critical role in regulating the metabolism, proliferation, and differentiation of intestinal epithelial cells. These processes are critical for epithelial barrier renewal, water, and ion balance (6-8). Research studies have shown that the GUCY2C signaling axis functions to suppress intestinal tumorigenesis, in part, by restraining AKT activity (9,10). Lesions in the GUCY2C signaling axis that frequently contribute to cellular transformation are associated with a loss of expression of its endogenous ligands, which promotes a compensatory increase in GUCY2C expression. Indeed, GUCY2C expression is maintained in a high percentage of primary and metastatic human colorectal tumors, which has raised interest in therapeutically targeting this receptor (11,12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SignalStain is a registered trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.