Render Target: STATIC
Render Timestamp: 2025-03-21T11:20:12.102Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:10.060
Product last modified at: 2025-03-12T12:30:46.325Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HADHA (F9M4C) Rabbit mAb #50257

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HADHA (F9M4C) Rabbit mAb recognizes endogenous levels of total HADHA protein. This antibody does not cross-react with HADHB protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val168 of human HADHA protein.

    Background

    Mitochondrial trifunctional enzyme, also known as mitochondrial trifunctional protein (MTP), catalyzes three reactions in the fatty acid β-oxidation pathway. It has two different types of subunits: α/HADHA and β/HADHB. HADHA possesses the 2,3-enoyl-CoA hydratase (ECH) and 3-hydroxyacyl-CoA dehydrogenase (HACD) activities, while HADHB has 3-ketoacyl-CoA thiolase activity (1-3). Studies show that HADHA stimulates ketone body production through fatty acid β-oxidation. β-hydroxybutyrate, one of the ketone bodies, inhibits gluconeogenesis (1). Furthermore, studies have identified mutations in HADHB that cause MTP deficiency, an autosomal recessive disorder severely restricting long-chain fatty acid oxidation (4-6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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