HCN2 (D1C6I) Rabbit mAb #14957
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
HCN2 (D1C6I) Rabbit mAb recognizes endogenous levels of total HCN2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala751 of human HCN2 protein.
Background
The voltage-activated ion channel superfamily includes both cyclic nucleotide-gated (CNG) channels and hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels (1,2). The direct binding of the cyclic nucleotides cAMP and cGMP promotes the gating of these channels, from the closed to an open state. Research studies demonstrate that HCN channels are involved in repetitive firing, resting membrane potential, and dendritic integration in neurons (1,3). HCN proteins are found in homotetramers and heterotetramers composed of HCN1 and HCN2 subunits (4). Tetramerization of HCN proteins results from cAMP binding to the channel, which triggers the release of the tonic inhibition exerted by the cytoplasmic cyclic nucleotide binding domain on the channel pore (5). HCN channels expressed in dorsal root ganglia neurons may play a role in acute inflammatory pain (6). Cardiac HCN channels ensure electrical rhythmicity in cardiomyocytes (7).
- Craven, K.B. and Zagotta, W.N. (2006) Annu Rev Physiol 68, 375-401.
- Jan, L.Y. and Jan, Y.N. (1990) Nature 345, 672.
- Nolan, M.F. et al. (2003) Cell 115, 551-64.
- Ulens, C. and Tytgat, J. (2001) J Biol Chem 276, 6069-72.
- Lolicato, M. et al. (2011) J Biol Chem 286, 44811-20.
- Acosta, C. et al. (2012) PLoS One 7, e50442.
- Biel, M. et al. (2009) Physiol Rev 89, 847-85.
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