Render Target: STATIC
Render Timestamp: 2024-12-12T11:16:41.713Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:54:20.534
Product last modified at: 2024-09-30T08:01:48.442Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HCN2 (D1C6I) Rabbit mAb #14957

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HCN2 (D1C6I) Rabbit mAb recognizes endogenous levels of total HCN2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala751 of human HCN2 protein.

    Background

    The voltage-activated ion channel superfamily includes both cyclic nucleotide-gated (CNG) channels and hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels (1,2). The direct binding of the cyclic nucleotides cAMP and cGMP promotes the gating of these channels, from the closed to an open state. Research studies demonstrate that HCN channels are involved in repetitive firing, resting membrane potential, and dendritic integration in neurons (1,3). HCN proteins are found in homotetramers and heterotetramers composed of HCN1 and HCN2 subunits (4). Tetramerization of HCN proteins results from cAMP binding to the channel, which triggers the release of the tonic inhibition exerted by the cytoplasmic cyclic nucleotide binding domain on the channel pore (5). HCN channels expressed in dorsal root ganglia neurons may play a role in acute inflammatory pain (6). Cardiac HCN channels ensure electrical rhythmicity in cardiomyocytes (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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