Interested in promotions? | Click here >>
8358
Hedgehog Signaling Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Hedgehog Signaling Antibody Sampler Kit #8358

Reviews ()
Citations (0)
Hedgehog Signaling Antibody Sampler Kit: Image 1

Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb #2207.

Hedgehog Signaling Antibody Sampler Kit: Image 2

Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 construct, using PTCH1 (C53A3) Rabbit mAb #2468.

Hedgehog Signaling Antibody Sampler Kit: Image 3

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody #2470.

Hedgehog Signaling Antibody Sampler Kit: Image 4

Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb #2520.

Hedgehog Signaling Antibody Sampler Kit: Image 5

Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (V812) Antibody #2534.

Hedgehog Signaling Antibody Sampler Kit: Image 6

Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb.

Hedgehog Signaling Antibody Sampler Kit: Image 7

Immunoprecipitation of endogenous PTCH1 from 293 cell lysates using PTCH1 (C53A3) Rabbit mAb. Western blot detection was performed using the same antibody.

Hedgehog Signaling Antibody Sampler Kit: Image 8

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody.

Hedgehog Signaling Antibody Sampler Kit: Image 9

Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb.

Hedgehog Signaling Antibody Sampler Kit: Image 10

Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (C68H3) Rabbit mAb.

Hedgehog Signaling Antibody Sampler Kit: Image 11

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Hedgehog Signaling Antibody Sampler Kit: Image 12

Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 expression construct, using PTCH1 (C53A3) Rabbit mAb.

To Purchase # 8358T
Product # Size Price
8358T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Shh (C9C5) Rabbit mAb 2207 20 µl
  • WB
H R 19, (45 precursor) Rabbit IgG
PTCH1 (C53A3) Rabbit mAb 2468 20 µl
  • WB
  • IP
H 180-210 Rabbit IgG
PTCH2 (G1191) Antibody 2470 20 µl
  • WB
  • IP
H 130 Rabbit 
SUFU (C54G2) Rabbit mAb 2520 20 µl
  • WB
H M R Mk 54 Rabbit IgG
GLI1 (C68H3) Rabbit mAb 3538 20 µl
  • WB
  • IP
H 160 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

This sampler kit provides an economical means of evaluating key members of the Hedgehog signaling pathway. The kit contains enough primary and secondary antibody to perform two western miniblot experiments.

Specificity / Sensitivity

Shh (C9C5) Rabbit mAb detects endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation followed by Western blot analysis. PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein. SUFU (C54G2) Rabbit mAb detects endogenous levels of total SUFU protein. GLI1 (C68H3) Rabbit mAb detects endogenous levels of total GLI1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region (predicted to be intracellular) surrounding Gly1191 of human PTCH2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu53 of human Shh, Pro1307 of human PTCH1, Leu458 of human SUFU, or Gly420 of human GLI1.

Background

The evolutionarily conserved Hedgehog (Hh) signaling pathway plays critical roles in the regulation of patterning, growth, and cell migration during embryonic development and adult tissue homeostasis. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh), and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the Hh receptors (6-9). The general organization of the Hh pathway consists of a series of repressive interactions. In the absence of Hh proteins (off-state), PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) (1,2). In the off-state, SUFU (Suppressor of Fused), originally identified in Drosophila as a suppressor of the Fused (Fu) kinase (10), suppresses Hh signaling by regulating the localization of the transcription factors Gli and Ci (11,12). In Drosophila, SUFU may also positively regulate Hh signaling depending on SUFU protein levels and Hh signal intensity (13).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
  2. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-92.
  4. Adolphe, C. et al. (2004) Development 131, 5009-19.
  5. Pathi, S. et al. (2001) Mech Dev 106, 107-17.
  6. Stone, D.M. et al. (1996) Nature 384, 129-34.
  7. Chen, Y. and Struhl, G. (1996) Cell 87, 553-63.
  8. Motoyama, J. et al. (1998) Nat Genet 18, 104-6.
  9. Smyth, I. et al. (1999) Hum Mol Genet 8, 291-7.
  10. Pham, A. et al. (1995) Genetics 140, 587-98.
  11. Barnfield, P.C. et al. (2005) Differentiation 73, 397-405.
  12. Méthot, N. and Basler, K. (2000) Development 127, 4001-10.
  13. Dussillol-Godar, F. et al. (2006) Dev Biol 291, 53-66.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.