Render Target: STATIC
Render Timestamp: 2024-12-02T11:57:38.669Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:55:18.025
Product last modified at: 2024-10-11T23:00:09.564Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

HEF1/NEDD9 (2G9) Mouse mAb #4044

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 105, 115
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HEF1/NEDD9 (2G9) Mouse mAb detects endogenous levels of total HEF1/NEDD9 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues from the human HEF1/NEDD9 protein.

    Background

    Human enhancer of filamentation protein 1 (HEF1), also known as neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), is part of the Cas family of proteins, which include HEF1/NEDD9, p130Cas and Efs (1). HEF1 is a predominantly cytoplasmic protein, localizing to focal adhesions during interphase, and centrosomes and other parts of the mitotic apparatus during G2/M phase of the cell cycle (2). HEF1 is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion, motility, growth and apoptosis (1). Phosphorylation of HEF1 is induced by a number of factors, including FAK, TGF-β, PDGFR, Abl, and BCR-ABL, which leads to coordinate binding of multiple downstream effector proteins via 15 known SH2 domain-binding sites (1). HEF1 is a key regulator of cancer metastasis. It is required for the invasive activity of glioblastomas (3), is linked to the promotion of metastasis in melanoma (4), and is found to be up-regulated in lung cancer metastasis (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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