HER2 (Trastuzumab Biosimilar) Human mAb #10583
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Human IgG1 kappa |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
| Endotoxin | <0.1 EU/μg of antibody |
Product Usage Information
Formulation
Storage
Specificity / Sensitivity
HER2 (Trastuzumab Biosimilar) Human mAb was confirmed to bind to its intended target protein HER2 using flow cytometry.
Species Reactivity:
Human
Source / Purification
HER2 (Trastuzumab Biosimilar) Human mAb is produced at Cell Signaling Technology. Trastuzumab is an antibody directed against the extracellular region of human HER2.
Background
The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).
Limited Uses
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