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Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

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W IP   IF F   H Mk
REACTIVITY SENSITIVITY MW (kDa) SOURCE
60 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HEXIM1 Antibody recognizes endogenous levels of total HEXIM1 protein.


Species predicted to react based on 100% sequence homology: Bovine, Dog, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HEXIM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).


1.  Ouchida, R. et al. (2003) Genes Cells 8, 95-107.

2.  Michels, A.A. et al. (2004) EMBO J 23, 2608-19.

3.  Yik, J.H. et al. (2003) Mol Cell 12, 971-82.

4.  Buratowski, S. (2009) Mol Cell 36, 541-6.

5.  Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.

6.  Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.

7.  Wada, T. et al. (1998) EMBO J 17, 7395-403.

8.  Yamada, T. et al. (2006) Mol Cell 21, 227-37.

9.  Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.

10.  Ketchart, W. et al. (2011) Oncogene 30, 3563-9.

11.  Ogba, N. et al. (2008) Cancer Res 68, 7015-24.

12.  Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.


Entrez-Gene Id 10614
Swiss-Prot Acc. O94992


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

9064
HEXIM1 Antibody