HIPK2 Antibody #5091
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130-140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
HIPK2 activated in response to DNA damage, including UV radiation and chemotherapeutic drugs, phosphorylates p53 at Ser46 to promote the transcription of pro-apoptotic p53 target genes (5-7). In addition, HIPK2 interacts with a number of transcription factors that control developmental processes, tumor suppression and apoptosis (4). The kinase is regulated by both sumoylation (8) and ubiquitination (9,10). Ubiquitination and subsequent degradation of HIPK2 is inhibited by DNA damaging agents. Caspase-dependent cleavage of HIPK2 removes the inhibitory domain and results in enhanced HIPK2 activity (11).
- Kim, Y.H. et al. (1998) J Biol Chem 273, 25875-9.
- Rochat-Steiner, V. et al. (2000) J Exp Med 192, 1165-74.
- Arai, S. et al. (2007) FEBS Lett 581, 5649-57.
- Rinaldo, C. et al. (2007) Biochem Cell Biol 85, 411-8.
- Hofmann, T.G. et al. (2002) Nat Cell Biol 4, 1-10.
- D'Orazi, G. et al. (2002) Nat Cell Biol 4, 11-9.
- Di Stefano, V. et al. (2004) Exp Cell Res 293, 311-20.
- Kim, Y.H. et al. (1999) Proc Natl Acad Sci U S A 96, 12350-5.
- Choi, D.W. et al. (2008) J Biol Chem 283, 4682-9.
- Winter, M. et al. (2008) Nat Cell Biol 10, 812-24.
- Gresko, E. et al. (2006) EMBO J 25, 1883-94.
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