|56612T||1 Kit (7 x 20 microliters)||
|YAP (D8H1X) XP® Rabbit mAb 14074||20 µl||
||H M R Hm Mk||65-78||Rabbit IgG|
|TAZ (D3I6D) Rabbit mAb 70148||20 µl||
||H M||50||Rabbit IgG|
|Ajuba (D4D8P) Rabbit mAb 34648||20 µl||
||H Mk||55||Rabbit IgG|
|PTPN14 (D5T6Y) Rabbit mAb 13808||20 µl||
||H M R||160||Rabbit IgG|
|Merlin (D3S3W) Rabbit mAb 12888||20 µl||
||H M R Hm Mk||70||Rabbit IgG|
|MST1 (D8B9Q) Rabbit mAb 14946||20 µl||
||H M R||60||Rabbit IgG|
|FRMD6 (D8X3R) Rabbit mAb 14688||20 µl||
||H M R||78||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala200 of mouse TAZ, Phe608 of human FRMD6, Leu737 of human PTPN14, Gln470 of human merlin, Arg77 of human Ajuba, Pro9 of human MST1, and recombinant protein corresponding to the carboxy terminus of human YAP protein. The sequence region surrounding the YAP epitope is 100% conserved among all known isoforms of YAP.
The Hippo Signaling Pathway is an evolutionarily conserved signaling cascade that regulates cell, tissue and organ growth in response to diverse environmental cues, including cell density, mechanical stress, growth factors and cytokines, and metabolic signaling (1,2). In mammals, the core components of this pathway are YAP and TAZ, transcriptional co-activators that regulate the expression of target genes that control cell proliferation and survival (3). The transcriptional control of YAP/TAZ target genes is regulated through a complex kinase cascade involving mammalian sterile-20-like kinases (MST1/2), LATS1/2 kinases and key adaptor proteins. Phosphorylation of YAP and TAZ by LATS kinases results in cytoplasmic sequestration of YAP and TAZ, inhibiting transcriptional activation of their target genes. A number of proteins have been identified that function upstream of MST1/2 and LATS1/2 to influence LATS-mediated phosphorylation of YAP/TAZ. These include the tumor suppressor protein Merlin (4), the FERM-domain containing protein 6 (FRMD6/Willin) (5), the LIM-domain containing adaptor protein Ajuba (6,7), and the protein tyrosine phosphatase PTPN14 (8).
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