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R Recombinant
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HLA-F (F4B9F) Rabbit mAb #41228

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HLA-F (F4B9F) Rabbit mAb recognizes endogenous levels of total HLA-F protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln162 of human HLA-F protein.

    Background

    HLA class I histocompatibility antigen, alpha chain F (HLA-F) is a nonclassical member of the HLA class I family and has less genetic diversity than classical HLA molecules (1-4). HLA-F expression is primarily intracellular but has been observed on the surface in some contexts, including some activated immune cell subsets, virus-infected cells, invasive extravillous cytotrophoblasts, and various cancer types (5,6). HLA-F can be expressed on the surface as an open conformer monomer or dimer, or in a complex with β2-microglobulin and peptide. As an open conformer monomer, HLA-F is observed to interact with activating KIR3DS1 and inhibitory KIR3DL2 (7,8). The HLA-F/β2-microglobulin peptide complex interacts with inhibitory receptors ILT2 and ILT4 (9,10). It is hypothesized that high surface expression of HLA-F in tumors can be a mechanism of immune escape through interaction with inhibitory receptors on immune cells (5). In the context of viral infection, HLA-F interaction with activating KIR3DS1 on natural killer (NK) cells can result in NK cell degranulation and cytokine production (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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