HLA-G (E8N9C) XP® Rabbit mAb #79769
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30-40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #49508.
Protocol
Specificity / Sensitivity
HLA-G (E8N9C) Rabbit mAb recognizes endogenous levels of total HLA-G protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu102 of human HLA-G protein.
Background
HLA-G (HLA-G histocompatibility antigen, class I, G) is a non-classical MHC molecule expressed by trophoblasts in placenta, thymic epithelial cells, and some tumors. Alternative splicing leads to generation of at least six isoforms, four that are transmembrane proteins and two that are soluble (1-4). It is an inhibitory molecule involved in immune tolerance and escape, originally studied for its role in maternal tolerance of the fetus during pregnancy (1-5). HLA-G binds ILT2, ILT4, and KIR2DL4, playing a role in the regulation of natural killer, T, and monocyte/macrophage cells (4-5). Its involvement in evasion of immune response makes it a potential target for immunotherapy (6).
- Kovats, S. et al. (1990) Science 248, 220-3.
- Pazmany, L. et al. (1996) Science 274, 792-5.
- Fujii, T. et al. (1994) J Immunol 153, 5516-24.
- Shiroishi, M. et al. (2003) Proc Natl Acad Sci U S A 100, 8856-61.
- LeMaoult, J. et al. (2005) FASEB J 19, 662-4.
- Curigliano, G. et al. (2013) Clin Cancer Res 19, 5564-71.
Limited Uses
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