Render Target: STATIC
Render Timestamp: 2024-12-10T10:57:39.901Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:58:26.258
Product last modified at: 2024-09-30T08:01:22.555Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HLTF (E9H5I) Rabbit mAb #45965

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HLTF (E9H5I) Rabbit mAb recognizes endogenous levels of total HLTF protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala998 of human HLTF protein.

    Background

    Helicase-like transcription factor (HLTF), also known as RUSH-1 or SMARCA3, belongs to the SNF2/SWI2 family of ATP-dependent remodelers (1-3). HLTF has been shown to interact with ubiquitin enzyme complexes to promote Lys-63-linked polyubiquitination of PCNA, maintaining genomic stability during DNA damage (4,5). HLTF has double-stranded DNA translocation activity, which can restart replication forks at DNA lesions, providing tumor suppressive functions (6). The HLTF gene is frequently hypermethylated in many different tumor types (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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