HNF4γ (E4V2B) Rabbit mAb #15742
- WB
- ChIP
Supporting Data
| REACTIVITY | H R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:100 |
Storage
Protocol
Specificity / Sensitivity
HNF4γ (E4V2B) Rabbit mAb recognizes endogenous levels of total HNF4γ protein.
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val363 of human HNF4γ protein.
Background
Hepatocyte nuclear factor 4γ (HNF4γ) is a transcription factor that belongs to the orphan nuclear receptor superfamily (1). Studies of Hnf4g knockout mice show that HNF4γ is involved in the regulation of glucose homeostasis (2,3). In addition, HNF4γ expression was shown to be upregulated in lung cancer. Overexpressed HNF4γ stimulates lung cancer cell proliferation (1). Furthermore, an aberrant gastrointestinal-lineage transcriptome was identified in about 30% of castration-resistant prostate cancers. HNF4γ, along with HNF1α, regulates this transcriptome. HNF4γ generates and maintains enhancers at the gastrointestinal-lineage genes as a pioneer factor and mediates castration resistance (4).
Limited Uses
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