|REACTIVITY||H M R Mk|
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro500 of human hnRNP M protein.
Heterogenous nuclear ribonucleoprotein M (hnRNP M) belongs to a family of RNA-binding multi-protein complexes (hnRNP proteins) that facilitate pre-mRNA processing (1,2). hnRNP M binds to GU rich regions of RNA and exists as 4 isoforms named M1-M4 (2). Binding to pre-mRNA in early stages of spliceosome assembly, hnRNP M contributes to splicing events by inducing exon skipping as well as promoting exon inclusion (3,4). hnRNP M is expressed on the surface of Kupffer cells, and acts as a receptor for carcinoembryonic antigen (CEA), a biomarker for colorectal cancer metastasis to the liver (5-7). hnRNP M has also shown to be a key regulator of the innate immune response in macrophages, where it represses key inflammatory and antimicrobial genes (8,9).
UniProt ID: P52272
Entrez-Gene Id: 4670
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