hnRNP U (E2O1R) Rabbit mAb #34095
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
hnRNP U (E2O1R) Rabbit mAb recognizes endogenous levels of total hnRNP U protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val479 of human hnRNP U protein.
Background
hnRNP U, also known as scaffold attachment factor A (SAF-A) or SP120, was first described as a nuclear matrix protein that contributes to RNA metabolism (1-3). hnRNP U has been shown to bind pre-mRNAs and contributes to splicing by controlling U2 snRNP maturation (4). Furthermore, hnRNP U has been shown to bind chromatin to help regulate its structure during interphase (5). During prophase, hnRNP U becomes phosphorylated by Aurora-B, which evicts hnRNP U and the chromatin-associated RNAs bound to it, a process critical to proper chromosome alignment (6,7). hnRNP U plays a role in the chromatin localization of Xist RNA, and loss of hnRNP U in ES cells fail to achieve an inactivated X chromosome (8). hnRNP U has also been shown to play a key role in immunity, functioning as a viral dsRNA sensor. Upon viral recognition, hnRNP U oligomerizes and activates numerous antiviral genes, including IFNB1 (9). Loss of function variants of hnRNP U have been shown to cause early onset epilepsy and intellectual disorders (10,11).
- Kiledjian, M. and Dreyfuss, G. (1992) EMBO J 11, 2655-64.
- Fackelmayer, F.O. et al. (1994) Eur J Biochem 221, 749-57.
- Göhring, F. et al. (1997) EMBO J 16, 7361-71.
- Xiao, R. et al. (2012) Mol Cell 45, 656-68.
- Nozawa, R.S. et al. (2017) Cell 169, 1214-1227.e18.
- Sharp, J.A. et al. (2020) J Cell Biol 219, .
- Douglas, P. et al. (2015) Mol Cell Biol 35, 2699-713.
- Hasegawa, Y. et al. (2010) Dev Cell 19, 469-76.
- Cao, L. et al. (2019) Cell Host Microbe 26, 369-384.e8.
- Leduc, M.S. et al. (2017) Am J Med Genet A 173, 2680-2689.
- Bramswig, N.C. et al. (2017) Hum Genet 136, 821-834.
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