HP1α (F3K7M) Rabbit mAb #47573
- WB
- IP
- IF
- ChIP
- C&R
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Chromatin IP | 1:50 |
| CUT&RUN | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Maison, C. and Almouzni, G. (2004) Nat. Rev. Mol. Cell Biol. 5, 296-304.
- Minc, E. et al. (2000) Cytogenet. Cell Genet. 90, 279-284.
- Nielsen, A.L. et al. (2001) Mol. Cell 7, 729-739.
- Lachner, M. et al. (2001) Nature 410, 116-120.
- Bannister, A.J. et al. (2001) Nature 410, 120-124.
- Muchardt, C. et al. (2002) EMBO Rep. 3, 975-981.
- Yamamoto, K. and Sonoda, M. (2003) Biochem. Biophys. Res. Commun. 301, 287-292.
- Fuks, F. et al. (2003) Nucleic Acids Res. 31, 2305-2312.
- Murzina, N. et al. (1999) Mol. Cell 4, 529-540.
- Nielsen, S.J. et al. (2001) Nature 412, 561-565.
- Ogawa, H. et al. (2002) Science 296, 1132-1136.
- Minc, E. et al. (1999) Chromosoma 108, 220-234.
- Zhao, T. et al. (2001) J. Biol. Chem. 276, 9512-9518.
- Lomberk, G. et al. (2006) Nat. Cell Biol. 8, 407-415.
Limited Uses
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