HPK1 (E6D2F) Rabbit mAb #53453
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 97 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
HPK1 (E6D2F) Rabbit mAb recognizes endogenous levels of total HPK1 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly741 of human HPK1 protein.
Background
Hematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7).
- Liou, J. et al. (2000) Immunity 12, 399-408.
- Ling, S. et al. (1999) Mol. Cell. Biol. 19, 1359-1368.
- Arnold, R. et al. (2001) J. Biol. Chem. 276, 14675-14684.
- Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.
- Sauer, K. et al. (2001) J. Biol. Chem. 276, 45207-45216.
- Chen, Y. et al. (1999) Oncogene 18, 7370-7377.
- Schulze-Luehrmann, J. et al. (2002) Blood 100, 954-960.
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