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HSD3B1 (F7Q2L) Rabbit mAb

HSD3B1 (F7Q2L) Rabbit mAb #15230

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	42
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HSD3B1 (F7Q2L) Rabbit mAb recognizes endogenous levels of total HSD3B1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro154 of human HSD3B1 protein.

Background

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (HSD3B1), also known as 3β-hydroxysteroid dehydrogenase-1 (3βHSD1), is critical for steroid hormone biosynthesis (1,2). This enzyme catalyzes the rate-limiting step of extragonadal androgen synthesis. Studies show that glucosamine released from cancer-associated fibroblasts (CAFs) enhances O-GlcNAcylation in prostate cancer cells, activating HSD3B1/3βHSD1 expression mediated by the transcription factor Elk1. This increases de novo intratumoral androgen biosynthesis and leads to castration-resistant prostate cancer (CRPC) (2). In addition, the adrenal-permissive HSD3B1 (1245C) allele, which upregulates intratumoral androgen biosynthesis, augments resistance to radiotherapy in prostate cancer (3).

Database Links

UniProt ID: P14060

Entrez-Gene Id: 3283

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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