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HSP10 (F8X5Y) Rabbit mAb

HSP10 (F8X5Y) Rabbit mAb #53668

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	10
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HSP10 (F8X5Y) Rabbit mAb recognizes endogenous levels of total HSP10 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HSP10 protein.

Background

Impaired mitochondrial integrity and function lead to the activation of a transcriptional response known as the mitochondrial unfolded protein response (UPR^mt) (1). Mitochondrial 10 kDa heat shock protein (HSP10), also known as heat shock protein family E member 1 (HSPE1), is a mitochondria-specific protein chaperone and a component in the UPR^mt pathway. Studies show that Akt3 depletion in primary endothelial cells causes mitochondrial stress and leads to upregulated expression of HSP10 along with HSP60, another mitochondria-specific protein chaperone. This finding suggests a critical role of Akt3 in maintaining mitochondrial homeostasis (2). In addition, HSP10 and HSP60 chaperones were shown to be the most abundant mitochondrial proteins (3).

Database Links

UniProt ID: P61604

Entrez-Gene Id: 3336

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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