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HSP40 (C64B4) Rabbit mAb #4871

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HSP40 (C64B4) Rabbit mAb detects endogenous levels of total HSP40 protein. This antibody does not cross-react with other HSPs.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to Glu223 of human HSP40/Hdj1.

    Background

    HSP40 and HSP40-like proteins represent a large family of chaperone proteins that are homologous to E. coli DnaJ protein (1). These proteins are classified into three subtypes based on their structures. The common feature of the family is a conserved J domain, which is usually located at the amino terminus of proteins and responsible for their association with HSP70 (1,2). Human HSP40, also known as Hdj1, belongs to subtype II that contain a unique Gly/Phe-rich region (2). HSP40 family proteins bind unfolded proteins, prevent their aggregation, and then deliver them to HSP70 (2,3). Another major function of HSP40 is to stimulate ATPase activity of HSP70, which causes conformational change of the unfolded proteins (4,5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 or components of the protein degradation machinery such as CHIP and BAG-1, which either leads to protein folding or degradation, respectively (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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