Render Target: STATIC
Render Timestamp: 2024-10-11T09:57:34.039Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-10-01 22:02:14.522
Product last modified at: 2024-10-05T08:00:16.830Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HTRA1/PRSS11 (F5F1P) Rabbit mAb #44783

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 40-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50
    Immunofluorescence (Frozen) 1:100 - 1:200
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HTRA1/PRSS11 (F5F1P) Rabbit mAb recognizes endogenous levels of total HTRA1/PRSS11 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the serine protease and PDZ domains of human HTRA1/PRSS11 protein.

    Background

    High-temperature requirement A serine peptidase 1 (HTRA1, PRSS11) is a trypsin-like serine protease involved in neuronal maturation, protein degradation, apoptosis, and the response to cellular stress. HTRA family members contain N-terminal IGFBP and Kazal-like domains, a serine protease domain, and a C-terminal PDZ domain (1). HTRA1 can be secreted and autocatalyzed (2) or retained in the cell and processed to shorter cytoplasmic- and nuclear-localized forms (3). Ser328Ala mutants lack both proteolytic and autocatalytic activity (2). HTRA1 regulates the extracellular matrix (ECM) by degrading aggrecan, collagen, decorin, fibromodulin, and fibronectin (4,5), while also breaking down signaling molecules such as TGF-β family members (6,7) and FGF8 (8). In the brain, HTRA1 degrades β-Amyloid and aggregated fibrillar tau (9,10). Elevated HTRA1 can contribute to osteoarthritis (5,11) and age-related macular degeneration (12). HTRA1 loss induces EMT and breast cancer cell invasion (13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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