Buy 3 and get the 4th for FREE* | Learn More >>
62714
Human Immune Cell Phenotyping IHC Antibody Sampler Kit
Primary Antibodies

Human Immune Cell Phenotyping IHC Antibody Sampler Kit #62714

Reviews ()
Citations (0)

Immunohistochemical analysis of paraffin-embedded human tonsil using CD3ε (D7A6E™) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human appendix using CD8α (C8/144B) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using FoxP3 (D2W8E™) (IHC Specific) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD11b/ITGAM (D6X1N) Rabbit mAb performed on the Leica® Bond™ Rx.

Flow cytometric analysis of Jurkat cells (blue) and THP-1 cells (green) using CD68 (D4B9C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil using CD68 (D4B9C) XP® Rabbit mAb.

Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 24 hr) followed by 24 hr without TPA, (left, positive) or HeLa cells (right, negative), using CD11c (D3V1E) XP® Rabbit mAb #45581 (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CD11c (D3V1E) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (D4V4B) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Flow cytometric analysis of MCF7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

Confocal immunofluorescent analysis of SH-SY5Y cells (left, positive) or HeLa cells (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Flow cytometric analysis of HeLa cells (blue) and SH-SY5Y cells (green) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymph node using CD8α (C8/144B) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using CD11b/ITGAM (D6X1N) Rabbit mAb.

Flow cytometric analysis of live human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD68 (D4B9C) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of Neuro-2a cells (left, high-expressing) or C2C12 cells (right, low-expressing) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of mouse spinal cord (left), or rat brainstem (right) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red). Tissue was mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD3ε (D7A6E™) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD8α (C8/144B) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, untransfected (left) or transfected with human FoxP3 protein (right), using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using CD11b/ITGAM (D6X1N) Rabbit mAb.

Flow cytometric analysis of fixed and permeabilized human peripheral blood mononuclear cells co-stained with anti-human CD14 using CD68 (D4B9C) XP® Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD68 (D4B9C) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Raji cell pellet (right, negative) using CD11c (D3V1E) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using CD3ε (D7A6E™) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human spleen using CD8α (C8/144B) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using FoxP3 (D2W8E™) Rabbit mAb (IHC Specific).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11b/ITGAM (D6X1N) Rabbit mAb.

Confocal immunofluorescent analysis of THP-1 (left) and Jurkat (right) cells using CD68 (D4B9C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Projected images from a z-stack series are shown.

Immunohistochemical analysis of paraffin-embedded THP-1 (left) and Jurkat (right) cell pellets using CD68 (D4B9C) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11c (D3V1E) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (D4V4B) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded mouse ovary using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded MOLT-4 (left) and PC-3 (right) cell pellets using CD3ε (D7A6E™) XP® Rabbit mAb.

Western blot analysis of extracts from various cells using CD11b/ITGAM (D6X1N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD68 (D4B9C) XP® rabbit mAb (red), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), Pan-keratin (C11) mouse mAb #4545 (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using CD11c (D3V1E) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (D4V4B) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (left) or NCAM (CD56) (123C3) Mouse mAb #3576 (right). These two antibodies detect independent, unique epitopes on human NCAM1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma with staining of peripheral nerve using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CD68 (D4B9C) XP® rabbit mAb (orange), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), PD-L2 (D7U8C™) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M™) XP® rabbit mAb #93668 (green), IDO (D5J4E™) rabbit mAb #86630 (yellow), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD11c (D3V1E) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (D4V4B) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded SH-SY5Y cell pellet (left, positive) or MCF7 cell pellet (right, negative) using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Western blot analysis of extracts from MOLT-4, HuT 102, and PC-3 cells using CD3ε (D7A6E™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from various cell lines using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

Western blot analysis of extracts from THP-1, Raji, or Jurkat cells using CD11c (D3V1E) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded mouse colon using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse prostate using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb.

Immunoprecipitation of NCAM1 protein from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NCAM1 (CD56) (E7X9M) XP® Rabbit mAb. Western blot analysis was performed using NCAM1 (CD56) (123C3) Mouse mAb #3576. Anti-mouse IgG, HRP-linked Antibody #7076 was used as the secondary antibody.

Western blot analysis of extracts from various cell lines and tissues using NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 62714T
Product # Size Price
62714T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD3ε (D7A6E™) XP® Rabbit mAb 85061 20 µl
  • WB
  • IP
  • IHC
H 23 Rabbit IgG
CD8α (C8/144B) Mouse mAb (IHC Specific) 70306 20 µl
  • IHC
H Mouse IgG1
FoxP3 (D2W8E™) Rabbit mAb (IHC Specific) 98377 20 µl
  • IHC
H 45 Rabbit IgG
CD11b/ITGAM (D6X1N) Rabbit mAb 49420 20 µl
  • WB
  • IHC
H 170 Rabbit IgG
CD68 (D4B9C) XP® Rabbit mAb 76437 20 µl
  • IHC
  • IF
  • F
H Rabbit IgG
CD11c (D3V1E) XP® Rabbit mAb 45581 20 µl
  • WB
  • IHC
  • IF
H 145 Rabbit IgG
CD19 (D4V4B) XP® Rabbit mAb 90176 20 µl
  • WB
  • IP
  • IHC
H M 95 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
NCAM1 (CD56) (E7X9M) XP® Rabbit mAb 99746 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 120 to 220 Rabbit IgG

Product Description

The Human Immune Cell Phenotyping IHC Antibody Sampler Kit provides an economical means of detecting the accumulation of immune cell types in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody included in the Human Immune Cell Phenotyping IHC Antibody Sampler Kit recognizes endogenous levels of its target human protein. CD11c (D3V1E) XP® Rabbit mAb does not cross-react with CD11b. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13, and 18. Pan-Keratin (C11) Mouse mAb does not cross-react with other keratins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Glu178 of human CD3ε protein, Leu427 of human CD19 protein, Pro799 of human NCAM1/CD56 protein, near the carboxy terminus of human CD8 protein, or with recombinant protein specific to human FoxP3, human CD68, human CD11c, or the amino terminus of human CD11b/ITGAM protein. Pan-Keratin (C11) Mouse mAb (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A431 cells.

Background

Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T cells that directly associates with the T cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of the TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). CD8 is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (2). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for CD4+ T regulatory cells (Tregs) (4). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (5). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (6). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (7,8). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (7-9). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (10,11). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (12,13). NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats (14). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (15). Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (16,17). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (16).

  1. Kuhns, M.S. et al. (2006) Immunity 24, 133-139.
  2. Zamoyska, R. (1994) Immunity 1, 243-6.
  3. Shortman, K. and Heath, W.R. (2010) Immunol Rev 234, 18-31.
  4. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  5. Solovjov, D.A. et al. (2005) J Biol Chem 280, 1336-45.
  6. Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
  7. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  8. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  9. Ramprasad, M.P. et al. (1995) Proc Natl Acad Sci U S A 92, 9580-4.
  10. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  11. Qualai, J. et al. (2016) PLoS One 11, e0154253.
  12. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  13. Scheuermann, R.H. and Racila, E. (1995) Leuk Lymphoma 18, 385-97.
  14. Cunningham, B. A. et al. (1987) Science 236, 799-806.
  15. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-2438.
  16. Moll, R. et al. (1982) Cell 31, 11-24.
  17. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
D2W8E is a trademark of Cell Signaling Technology. Inc.
D7A6E is a trademark of Cell Signaling Technology. Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
DRAQ5 is a registered trademark of Biostatus Limited.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
ProLong is a registered trademark of Life Technologies Corporation.
Tween is a registered trademark of ICI Americas, Inc.