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Human TNF-α Neutralizing (D1B4) Rabbit mAb #7321

Inquiry Info. # 7321

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. TNF-α has known functions of cell cytotoxicity, cell activation, and apoptosis in different cell types. Human TNF-α Neutralizing (D1B4) Rabbit mAb has been shown to neutralize the cytotoxic effects of TNF-α in L-929 mouse fibroblast cells. Utilizing 1 ng/ml of hTNF-α #8902 and 1 µg/ml of actinomycin D, Human TNF-α Neutralizing (D1B4) Rabbit mAb rescued L-929 cells with an ND50 in the range of 4-12 ng/ml.
    Endotoxin <0.1 EU/µg of antibody

    Product Usage Information

    CST recommends incubation of the neutralizing antibody with intended target for 2 hours at 37ºC before addition to the experiment at an optimal concentration determined by the user.

    Reconstitution: Add 0.2 ml sterile dH2O to a final concentration of 0.5 mg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

    Formulation

    Lyophilized from a 0.2 µm filtered solution in HEPES with trehalose.

    Storage

    Store lyophilized material at -20ºC. After reconstitution, recommended storage at 4ºC for 1 month or -20ºC for 6 months. Avoid repeated freeze/thawing.

    Specificity / Sensitivity

    Human TNF-α Neutralizing (D1B4) Rabbit mAb binds to human TNF-α and neutralizes its cytotoxic effects. This antibody does not cross-react with mouse TNF-α.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human TNF-α protein.

    Background

    TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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