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91473
IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

IDO (D5J4E) Rabbit mAb (BSA and Azide Free) #91473

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using IDO (D5J4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IDO (D5J4E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IDO (D5J4E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using IDO (D5J4E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IDO (D5J4E) Rabbit mAb (yellow), PD-L1 (E1L3N®) XP® Rabbit mAb #13684 (red), PD-L2 (D7U8C) XP®Rabbit mAb (magenta) #82723, Arginase-1 (D4E3M) XP® Rabbit mAb #93668 (green), CD68 (D4B9C) XP®Rabbit mAb #76437 (orange), and Pan-keratin (C11) Mouse mAb #4545 (cyan). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; right), using IDO (D5J4E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: IDO (D5J4E™) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, overnight; right), using IDO (D5J4E) Rabbit mAb (green) and DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 91473
Cat. # Size Qty. Price
91473SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 43
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #86630. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #86630.

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IDO (D5J4E™) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total IDO (IDO-1, INDO) protein. The antibody does not cross-react with IDO-2 (INDOL1). Some nonspecific staining of normal breast epithelium has been observed.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IDO protein.

Background

INDO/IDO1/indoleamine 2,3-dioxygenase (IDO) is an IFN-γ-inducible enzyme that catalyzes the rate-limiting step of tryptophan degradation (1). IDO is upregulated in many tumors and in dendritic cells in tumor-draining lymph nodes. Elevated tryptophan catabolism in these cells leads to tryptophan starvation of T cells, limiting T cell proliferation and activation (2). Therefore, IDO is considered an immunosuppresive molecule, and research studies have shown that upregulation of IDO is a mechanism of cancer immune evasion (3). The gastrointestinal stromal tumor drug, imatinib, was found to act, in part, by reducing IDO expression, resulting in increased CD8+ T cell activation and induction of apoptosis in regulatory T cells (4). In addition to its enzymatic activity, IDO was recently shown to have signaling capability through an immunoreceptor tyrosine-based inhibitory motif (ITIM) that is phosphorylated by Fyn in response to TGF-β. This leads to recruitment of SHP-1 and activation of the noncanonical NF-κB pathway (5).
  1. Yasui, H. et al. (1986) Proc Natl Acad Sci U S A 83, 6622-6.
  2. Mellor, A.L. et al. (2003) Adv Exp Med Biol 527, 27-35.
  3. Prendergast, G.C. (2008) Oncogene 27, 3889-900.
  4. Balachandran, V.P. et al. (2011) Nat Med 17, 1094-100.
  5. Pallotta, M.T. et al. (2011) Nat Immunol 12, 870-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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