Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using IGFBP7 (E3Z8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human kidney using IGFBP7 (E3Z8L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using IGFBP7 (E3Z8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using IGFBP7 (E3Z8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using IGFBP7 (E3Z8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse pancreas using IGFBP7 (E3Z8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded SCaBER cell pellet (left, positive) or HT-29 cell pellet (right, negative) using IGFBP7 (E3Z8L) XP® Rabbit mAb.
|Immunohistochemistry (Paraffin)||1:50 - 1:200|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 283
IGFBP7 (E3Z8L) XP® Rabbit mAb recognizes endogenous levels of total IGFBP7 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu241 of human IGFBP7 protein.
IGFBP7 (also known as Mac25, TAF, or IGFBP-rP1) belongs to the IGFBP superfamily, which plays an integral role in regulating insulin-like growth factor (IGF) actions in a wide variety of cell types. There are six known high-affinity IGF binding family members (IGFBP1-6), and ten low-affinity IGF binding members. These family members are structurally related, but encoded by distinct genes (1,2). IGFBP7 is a low-affinity IGF binding protein (1). The protein functions through its binding to secreted growth factors including IGF1, insulin, and activin (3,4). IGFBP7 levels have been related to cancer development and tissue injury. Loss of expression of IGFBP7 has been associated with poor survival in multiple cancer types (5,6) and with tumor chemotherapy resistance (7,8). IGFBP7 also has been identified as a cell cycle arrest biomarker for human acute kidney injury (AKI) and serves as a prognostic indicator for early stage AKI development (9-11).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.