IκBε Antibody #9249
- WB
Inquiry Info. # 9249
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Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Description
| MW (kDa) | 40 |
| Activity | ****** The range listed in PDP for this antigen is 59-70. This does not match the most commonly identified human entries in a BLAST search (for most BLAST returns the antigen sequence matched Human IkB epsilon 199-209). The 59-70 notation does not correlate with other standard database entries for human IkB epsilon - including the uniprot enrty (http://www.uniprot.org/uniprot/O00221). For this antigen the correct notation is residues 199-209, residues 59-70 (as currently listed) is incorrect. Note Q198 has been substituted with a cysteine in the immunizing antigen (Q198C, thus 198-208?). Also note the webpage and datasheet state: "Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the middle of human IκBε". This information is correct and consistent with the antigen at residues 199-209. The peptide notebook will remain incorrect. |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The regulation of IκBβ and IκBε is similar to that of IκB-α. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκB-β occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Hoffmann, A. et al. (2002) Science 298, 1241-5.
- Shirane, M. et al. (1999) J Biol Chem 274, 28169-74.
Alternate Names
I-kappa-B-epsilon; IkappaBepsilon; IkB-E; IkB-epsilon; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFKB inhibitor epsilon; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
Limited Uses
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