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  3. IKKε Antibody

IKKε Antibody #2686

Inquiry Info. # 2686

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 80
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    IKKε Antibody detects endogenous levels of total IKKepsilon protein. This antibody does not cross-react with other IKKs, but may cross-react with TBK1/NAK.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequences of human IKKepsilon. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
    Recently, two homologs of IKKalpha and IKKbeta have been described, called IKKepsilon (also known as IKK-i) and TBK-1 (also known as T2K or NAK), and activation of either of these kinases results in NFkappaB activation. IKKepsilon contains the kinase domain in its amino-terminus, which shares 30% identity to that of IKKalpha or IKKbeta. IKKepsilon is expressed mainly in immune cells, and may play a special role in the immune response (14-18).
    1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
    2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
    3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
    4. Brown, K. et al. (1995) Science 267, 1485-8.
    5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
    6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
    7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
    8. Zandi, E. et al. (1997) Cell 91, 243-52.
    9. Karin, M. (1999) Oncogene 18, 6867-74.
    10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
    11. Mercurio, F. et al. (1997) Science 278, 860-6.
    12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
    13. Delhase, M. et al. (1999) Science 284, 309-13.
    14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
    15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
    16. Tojima, Y. et al. (2000) Nature 404, 778-82.
    17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
    18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.

    Alternate Names

    I-kappa-B kinase epsilon; IKBKE; IKK-E; IKK-epsilon; IKK-i; IKK-related kinase epsilon; IKKE; IKKI; Inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa B kinase subunit epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297

    Database Links

    UniProt ID: Q14164


    Entrez-Gene Id: 9641


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