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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. IKKε (D61F9) XP® Rabbit mAb (BSA and Azide Free)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

IKKε (D61F9) XP® Rabbit mAb (BSA and Azide Free) #96794

Filter:
  • WB
  • IF
  • F

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #3416. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    Formulation

    Supplied in 1X PBS, BSA and Azide Free.

    For standard formulation of this product see product #3416.

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    IKKε (D61F9) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of total IKKε protein.


    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of mouse IKKε.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
    Recently, two homologs of IKKα and IKKβ have been described, called IKKε (also known as IKK-i) and TBK-1 (also known as T2K or NAK), and activation of either of these kinases results in NF-κB activation. IKKε contains the kinase domain in its amino terminus, which shares 30% identity to that of IKKα or IKKβ. IKKε is expressed mainly in immune cells, and may play a special role in the immune response (14-18).

    1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
    2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
    3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
    4. Brown, K. et al. (1995) Science 267, 1485-8.
    5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
    6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
    7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
    8. Zandi, E. et al. (1997) Cell 91, 243-52.
    9. Karin, M. (1999) Oncogene 18, 6867-74.
    10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
    11. Mercurio, F. et al. (1997) Science 278, 860-6.
    12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
    13. Delhase, M. et al. (1999) Science 284, 309-13.
    14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
    15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
    16. Tojima, Y. et al. (2000) Nature 404, 778-82.
    17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
    18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.

    Database Links

    UniProt ID: Q14164


    Entrez-Gene Id: 9641


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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