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IL1F10 (F5U1T) Rabbit Monoclonal Antibody #60507

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 17
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IL1F10 (F5U1T) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL1F10 protein. This antibody detects a nonspecific band at 60 kDa in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human IL1F10 protein.

    Background

    The interleukin-1 (IL-1) family of cytokines is critical in regulating innate and adaptive immune responses. The IL-1 family consists of receptor agonists and antagonists that have either pro- or anti-inflammatory roles, respectively (1-4). Interleukin 1 family member 10 (IL1F10), also known as IL-38, is a member of the IL-1 family that was originally shown to suppress inflammatory reactions in human cell lines and mouse models (2,3). IL1F10 is expressed in the heart, tonsil, skin, thymus, placenta, and particularly in keratinocytes and B cells (5). IL1F10 can induce a general anti-inflammatory program in several cell types, such as epithelial cells, dendritic cells (DCs), myeloid cells, Th17 cells, and regulatory T cells (Tregs) (3,5). Elevated IL1F10 expression in immune cells is indicative of its role in modulating immune cell function, and its expression in keratinocytes and epithelial cells suggests a function in maintaining immunological stability at sites of infection or environmental exposure. Recent work has focused on the role of dysregulated IL1F10 in cancer and autoimmunity (3,4,6,8). In mouse models, exogenous IL1F10 had a protective effect against the development of rheumatoid arthritis (RA), whereas its depletion worsened the disease in vivo (7). Aberrant expression of IL1F10 has been shown in several cancers, including colorectal, lung, and prostate cancers, making it a potential chemotherapeutic target (8).

    Alternate Names

    Family of interleukin 1-theta; FIL1 theta; FIL1-theta; FIL1T; FKSG75; IL-1 theta; IL-1F10; IL-1F10 (canonical form IL-1F10a); IL-1HY2; IL-38; IL1-theta; IL1F10; IL1FA; IL1HY2; IL38; interleukin 1 family member 10; interleukin 1 family member 10 (theta); interleukin 1 family, member 10 (theta); Interleukin-1 family member 10; Interleukin-1 HY2; interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta; Interleukin-1 theta; Interleukin-38; MGC119831; MGC119832; MGC119833

    For Research Use Only. Not for Use in Diagnostic Procedures.
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