For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL1RAP protein.
Interleukin-1 receptor accessory protein (IL1RAP/IL1R3) is a type-I transmembrane glycoprotein and component of the IL-1 and IL-33 receptor signaling complexes (1,2). Research studies have demonstrated that IL1RAP expression is required for IL-1-mediated signaling through its ability to recruit IRAK to the IL-1R complex (3-7). In the context of human disease, research studies have shown that IL1RAP is overexpressed on the surface of AML stem cells and associates with c-KIT and FLT3 to mediate pro-proliferative and pro-survival signaling events downstream of these oncogenic receptor tyrosine kinases (8,9). As such, IL1RAP has emerged as a novel immuno-therapeutic target for AML and other malignancies of the myeloid compartment (10,11).
UniProt ID: Q9NPH3
Entrez-Gene Id: 3556
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