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Render Timestamp: 2025-03-21T10:58:00.672Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:17.899
Product last modified at: 2025-02-05T13:00:22.265Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Influenza A Nucleoprotein (NP) (F8L6X) Rabbit mAb #99797

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Endogenous
    MW (kDa) 56
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Influenza A Nucleoprotein (NP) (F8L6X) Rabbit mAb recognizes endogenous levels of total influenza A virus (Russia:St.Petersburg/8/2006 H1N1) nucleoprotein. This antibody cross-reacts with nucleoprotein from H1N1, H3N2, and H5N1, and will broadly react with influenza A subtypes.

    Species Reactivity:

    Virus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of influenza A virus (Russia:St.Petersburg/8/2006 H1N1) nucleoprotein (Uniprot #B4URE0).

    Background

    Influenza, or flu, is a contagious respiratory virus of the Orthomyxoviridae family, which is subdivided into four genera: A, B, C, and D (1). Influenza A is the most common flu virus causing infections in humans and is the source of seasonal epidemics and occasional pandemics (2). The main proteins encased in the viral nucleocapsid are hemagglutinin (H) and neuraminidase (N), from which viral subtype names are derived (3,4). Influenza A subtypes H1N1 and H3N2 are the source of seasonal infections in humans and are regularly vaccinated against (2). The influenza A nucleoprotein (NP) plays a key role in the viral replication and lifecycle. NP forms ribonucleoprotein complexes that are essential for the transcription and replication of the virus. As the NP is highly conserved across influenza A subtypes, it has become a target for both vaccine development and antiviral drug development (5,6). Host protein ZBP1 directly binds to viral NP, inducing necroptosis of infected cells via the RIPK3 pathway (7). NP can inhibit the innate immune response by binding to mitochondrial antiviral signaling protein (MAVS) and Toll-interacting protein (Tollip), activating mitophagy and subsequent MAVS degradation (8).
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