Render Target: STATIC
Render Timestamp: 2024-12-03T11:26:06.758Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-11-26 23:01:10.438
Product last modified at: 2024-12-02T09:00:11.163Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

INO80 (F2I3E) Rabbit mAb #73828

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 280
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    INO80 (F2I3E) Rabbit mAb recognizes endogenous levels of total INO80 protein. This antibody detects bands of unknown origin at 145, 55, and 50 kDa.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1547 of human INO80 protein.

    Background

    The INO80 complex is an ATP-dependent chromatin remodeling complex critical for many functions, including transcriptional regulation and DNA repair. INO80 is a large multi-subunit complex consisting of the key ATPase INO80 along with RUVBL1, RUVBL2, ARP4/5/8, and others (1-3). INO80 prefers binding to hexasomes and can facilitate the exchange of H2A.Z/H2B dimers with H2A/H2B (4-6). The INO80 complex is recruited to double-strand breaks (DSBs) through interaction between gamma-H2AX and complex member NHP10 (7,8). INO80 also plays an important role in transcription as it is required for proper pausing of RNA polymerase II (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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