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Integrin α9β1 (Y9A2) Mouse mAb #4703

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Inquiry Info. # 4703

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 150: alpha9, 130: beta1
    Source/Isotype Mouse IgG1
    Application Key:
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Integrin α9β1 (Y9A2) Mouse mAb detects endogenous levels of total α9/β1 integrin heterodimer.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with murine L cells transfected with human α9 integrin protein.

    Background

    Integrins are transmembrane glycoproteins that form heterodimers consisting of one α and one β subunit. The dimers act as receptors for extracellular matrix (ECM) proteins at sites of cell adhesion, and interact with focal adhesion (FA) proteins on the cytosolic side, forming the connection between the ECM and the actin cytoskeleton. Signaling to and from integrins regulates cell adhesion, motility, proliferation, apoptosis and gene expression, impacting cellular processes such as development, wound healing, immune response, invasion, metastasis and angiogenesis (reviewed in 1,2). α9β1 integrin is expressed in epithelial cells, smooth and skeletal muscle, neutrophils and hepatocytes (3). Its ligands include the ECM protein tenascin (4) and vascular cell adhesion molecule-1 (VCAM-1) (5). The cytoplasmic domain of α9 integrin binds the focal adhesion adaptor protein, paxillin, inhibiting cell spreading (6,7). Binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9/β1 enhancement of cell migration (8). Physiological functions include development of the lymphatic system (9), possibly through binding to the lymphatic vascular endothelial growth factors VEGF-C and -D (10), neutrophil migration (5), and myogenic differentiation (11).

    Alternate Names

    ALPHA-RLC; CD29; fibronectin receptor beta subunit; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; GPIIA; Integrin alpha-9; Integrin alpha-RLC; integrin beta 1; Integrin beta-1; Integrin beta1; integrin subunit alpha 9; integrin subunit beta 1; integrin VLA-4 beta subunit; integrin, alpha 4-like; integrin, alpha 9; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITA9; ITB1; ITGA4L; ITGA9; ITGB1; MDF2; MSK12; RLC; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB

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