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4749
Integrin Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Integrin Antibody Sampler Kit #4749

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Integrin Antibody Sampler Kit: Image 1

Western blot analysis of extracts from A549, NIH/3T3 and COS cell lines using Integrin α5 Antibody.

Integrin Antibody Sampler Kit: Image 2

Western blot analysis of extracts from A549 and C6 cells using Integrin αV Antibody.

Integrin Antibody Sampler Kit: Image 3

Flow cytometric analysis of fixed and permeabilized A-204 cells (blue, negative) and A-431 cells (green, positive) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Integrin Antibody Sampler Kit: Image 4

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 5

Flow cytometric analysis of fixed and permeabilized Jurkat cells using Integrin α4 (D2E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Integrin Antibody Sampler Kit: Image 6

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β1 (D2E5) Rabbit mAb.

Integrin Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of A549 cells using Integrin β5 (D24A5) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Integrin Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Integrin Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Integrin Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded cell pellets, U-87 MG (left) or SNB19 (right), using Integrin β1 (D2E5) Rabbit mAb.

Integrin Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 13

Western blot analysis of extracts from various cell lines using Integrin β5 (D24A5) Rabbit mAb.

Integrin Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 15

Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Integrin β1 (D2E5) Rabbit mAb.

Integrin Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human testes using Integrin β3 (D7X3P) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Integrin Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human skin using Integrin β1 (D2E5) Rabbit mAb.

Integrin Antibody Sampler Kit: Image 20

Western blot analysis of extracts from U-87 MG, HUVEC and C2C12 cell lines using Integrin β3 (D7X3P) XP® Rabbit mAb.

Integrin Antibody Sampler Kit: Image 21

Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Integrin Antibody Sampler Kit: Image 22

Western blot analysis of extracts from various cell lines using Integrin β1 (D2E5) Rabbit mAb.

To Purchase # 4749T
Product # Size Price
4749T
1 Kit  (7 x 20 µl) $ 500

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Integrin α5 Antibody 4705 20 µl
  • WB
H M Mk 150 Rabbit 
Integrin αV Antibody 4711 20 µl
  • WB
  • IP
H R 135, 140 Rabbit 
Integrin β4 (D8P6C) XP® Rabbit mAb 14803 20 µl
  • WB
  • IHC
  • F
H 210 Rabbit IgG
Integrin α4 (D2E1) XP® Rabbit mAb 8440 20 µl
  • WB
  • IP
  • IF
  • F
H M R 70, 140, 150, Rabbit IgG
Integrin β1 (D2E5) Rabbit mAb 9699 20 µl
  • WB
  • IHC
H 115, 135 Rabbit IgG
Integrin β3 (D7X3P) XP® Rabbit mAb 13166 20 µl
  • WB
  • IHC
H M 100 Rabbit IgG
Integrin β5 (D24A5) Rabbit mAb 3629 20 µl
  • WB
  • IP
  • IF
H M R Mk 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Integrin Antibody Sampler Kit provides an economical means to screen samples for α and β subunits of integrin molecules. The kit includes enough primary and secondary antibody to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

All antibodies in the Integrin Antibody Sampler Kit detect endogenous levels of their target α and β integrin subunits of the integrin dimer. None of the antibodies provided detect intact dimers or their activational state.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser458 of human integrin α5 protein, Arg108 of human integrin αV. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide sequence corresponding to residues surrounding Ser1027 of human integrin a4 protein, the carboxy terminus of human integrin β4 protein, Pro680 of human integrin b1 protein, Ile114 of human integrin b3 protein, and Lys789 of human β5 protein.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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