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4749
Integrin Antibody Sampler Kit

Integrin Antibody Sampler Kit #4749

Western Blotting Image 1

Western blot analysis of extracts from A549, NIH/3T3 and COS cell lines using Integrin α5 Antibody.

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Western Blotting Image 2

Western blot analysis of extracts from A549 and C6 cells using Integrin αV Antibody.

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Western Blotting Image 3

Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP® Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using Integrin β1 (D2E5) Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of extracts from U-87 MG, HUVEC and C2C12 cell lines using Integrin β3 (D7X3P) XP® Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of extracts from various cell lines using Integrin β5 (D24A5) Rabbit mAb.

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Flow Cytometry Image 10

Flow cytometric analysis of Jurkat cells using Integrin α4 (D2E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β1 (D2E5) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

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IF-IC Image 13

Confocal immunofluorescent analysis of A549 cells using Integrin β5 (D24A5) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb.

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IF-IC Image 15

Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 16

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Integrin β1 (D2E5) Rabbit mAb.

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb.

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded human skin using Integrin β1 (D2E5) Rabbit mAb.

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded human testes using Integrin β3 (D7X3P) XP® Rabbit mAb.

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Flow Cytometry Image 21

Flow cytometric analysis of A-204 cells (blue) and A-431 cells (green) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Integrin α5 Antibody 4705 20 µl
  • WB
H M Mk 150 Rabbit 
Integrin αV Antibody 4711 20 µl
  • WB
  • IP
H R 135, 140 Rabbit 
Integrin β4 (D8P6C) XP® Rabbit mAb 14803 20 µl
  • WB
  • IHC
  • F
H 210 Rabbit IgG
Integrin α4 (D2E1) XP® Rabbit mAb 8440 20 µl
  • WB
  • IP
  • IF
  • F
H M R 70, 140, 150, Rabbit IgG
Integrin β1 (D2E5) Rabbit mAb 9699 20 µl
  • WB
  • IHC
H 115, 135 Rabbit IgG
Integrin β3 (D7X3P) XP® Rabbit mAb 13166 20 µl
  • WB
  • IHC
H M 100 Rabbit IgG
Integrin β5 (D24A5) Rabbit mAb 3629 20 µl
  • WB
  • IP
  • IF
H M R Mk 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Integrin Antibody Sampler Kit provides an economical means to screen samples for α and β subunits of integrin molecules. The kit includes enough primary and secondary antibody to perform two Western blot experiments with each antibody.

All antibodies in the Integrin Antibody Sampler Kit detect endogenous levels of their target α and β integrin subunits of the integrin dimer. None of the antibodies provided detect intact dimers or their activational state.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser458 of human integrin α5 protein, Arg108 of human integrin αV. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide sequence corresponding to residues surrounding Ser1027 of human integrin a4 protein, the carboxy terminus of human integrin β4 protein, Pro680 of human integrin b1 protein, Ile114 of human integrin b3 protein, and Lys789 of human β5 protein.

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
Entrez-Gene Id
3676 , 3685 , 3688 , 3690 , 3691 , 3693 , 3678
Swiss-Prot Acc.
P13612 , P06756 , P05556 , P05106 , P16144 , P18084 , P08648
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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