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  1. Products /
  2. Primary Antibodies /
  3. Integrin β7 (F3Z7J) Rabbit mAb
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Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Integrin β7 (F3Z7J) Rabbit mAb #29425

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  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800
    Flow Cytometry (Live) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Integrin β7 (F3Z7J) Rabbit mAb recognizes endogenous levels of total integrin β7 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu21 of human integrin β7 protein.

    Background

    Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling) but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Integrin β7 associates with integrin α4 and CD103 (ITGAE), forming the receptor complexes α4β7 and αEβ7 respectively. The α4β7 integrin receptor complex is expressed in CD4+ T cells, B cells, NK cells, eosinophils, basophils, macrophages, mast cells, and endothelial cells (4). αEβ7 integrin is expressed on mucosal T lymphocytes, binds to E-cadherin expressed on the gut epithelium, and promotes lymphocyte retention (5). The known α4β7 integrin receptor ligands are fibronectin, mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and vascular cell adhesion molecule-1 (VCAM-1) (6). Integrin β7 plays important roles in recruiting immune cells to the gastrointestinal tract and in the development of Peyer’s patches in the intestinal mucosa (7). The α4β7 integrin receptor complex plays a role in differentiation, survival, adhesion, and eosinophil and lymphocyte rolling (8). The dysfunction of integrin β7 and its receptor complexes is associated with inflammatory conditions such as inflammatory bowel disease (IBD), and integrins are emerging therapeutic targets for the treatment of IBD (9). Integrin β7 is also a therapeutic target for multiple myeloma (MM) and plays key roles in MM-cell migration, adhesion, and bone marrow homing (10).  

    Database Links

    UniProt ID: P26010


    Entrez-Gene Id: 3695


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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