Buy 3 Get a 4th Free* | Learn More >>
9092
StemLight™ iPS Cell Reprogramming Antibody Kit

StemLight™ iPS Cell Reprogramming Antibody Kit #9092

IF-IC Image 1

Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, Nanog (D73G4) XP® Rabbit mAb #4903, c-Myc (D84C12) XP® Rabbit mAb #5605, and LIN28A (D84C11) XP® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
IF-IC Image 2

Confocal immunofluorescent analysis of lentiviral transduced human fibroblast cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, c-Myc (D84C12) XP® Rabbit mAb #5605, and KLF4 Antibody #4038 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Western Blotting Image 3

Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 4

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 5

Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 6

Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 7

Western blot analysis of extracts from various cell lines using KLF4 antibody.

Learn more about how we get our images
Western Blotting Image 8

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) Rabbit mAb.

Learn more about how we get our images
Flow Cytometry Image 9

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.

Learn more about how we get our images
Flow Cytometry Image 11

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 12

Western blot analysis of extracts from various cell lines using c-Myc (D84C12) Rabbit mAb.

Learn more about how we get our images
IF-IC Image 13

Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem (mES) cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Learn more about how we get our images
Flow Cytometry Image 14

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.

Learn more about how we get our images
IF-IC Image 15

Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Flow Cytometry Image 16

Flow cytometric analysis of Raji cells using c-Myc (D84C12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Learn more about how we get our images
Flow Cytometry Image 17

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.

Learn more about how we get our images
IF-IC Image 18

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Learn more about how we get our images
IF-IC Image 19

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red).

Learn more about how we get our images
IF-IC Image 20

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Oct-4A (C30A3) Rabbit mAb 2840 20 µl
  • WB
  • IF
  • F
H M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb 3579 20 µl
  • WB
  • IF
  • F
H 35 Rabbit 
Nanog (D73G4) XP® Rabbit mAb 4903 20 µl
  • WB
  • IHC
  • IF
  • F
H 42 Rabbit IgG
LIN28A (D84C11) XP® Rabbit mAb 3695 20 µl
  • WB
  • IF
  • F
H 26 Rabbit IgG
KLF4 Antibody 4038 20 µl
  • WB
H M Mk 65 Rabbit 
c-Myc (D84C12) Rabbit mAb 5605 20 µl
  • WB
  • IF
  • F
H M R 57-65 Rabbit IgG

The StemLight™ iPS cell Reprogramming Antibody Kit contains a panel of antibodies for the detection of various proteins, combinations of which have been used to reprogram somatic cells to Induced Pluripotent Stem (iPS) cells. The kit can be used to track efficiency of expression of the reprogramming factors following transfection, viral transduction and other means of protein delivery. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications --please refer to individual data sheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.

Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein. Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total nanog protein. LIN28A (D84C11) XP® Rabbit mAb detects endogenous levels of total LIN28A protein. c-Myc (D84C12) Rabbit mAb detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276 or #2278). KLF4 Antibody detects endogenous levels of total KLF4 protein by western blot, but for immunofluorescence it is only recommended for detection of exogenously expressed KLF4 protein. All components of this kit have been validated for reactivity to human protein, however some components also have additional species cross reactivities. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A, a synthetic peptide corresponding to residues surrounding Gly179 of human Sox2, a synthetic peptide corresponding to residues near the amino terminus of human nanog protein, a synthetic peptide corresponding to residues near the carboxy terminus of human LIN28A, and a synthetic peptide corresponding

to residues near the amino terminus of c-Myc. Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to residues near the amino terminus of human KLF4. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.

Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.

SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neuraminadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  5. Okita, K. et al. (2007) Nature 448, 313-7.
  6. Yu, J. et al. (2007) Science 318, 1917-20.
  7. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37.
  8. Draper, J.S. et al. (2002) J Anat 200, 249-58.
  9. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

To Purchase # 9092T

Product Number Size Price
9092T 1 Kit (6 x 20 µl) $409.00.0
Quantity Subtotal
$0.00