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To Purchase # 9945T
| 9945T | 1 Kit (3 x 20 µl) | $239.00 |
| $0.00 | ||
Jak Isoform Sampler Kit #9945
Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines using Jak1 (6G4) Rabbit mAb.
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Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.
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Western blot analysis of extracts from various cell lines using Jak3 (D1H3) Rabbit mAb.
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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344, in the presence of control peptide (left) or Jak1 Blocking Peptide (right).
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Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.
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Confocal immunofluorescent analysis of KARPAS-299 cells (left) and A549 cells (right) using Jak3 (D1H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak1 (6G4) Rabbit mAb.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).
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Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
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Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
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| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Jak1 (6G4) Rabbit mAb 3344 | 20 µl |
|
H M R | 130 | Rabbit IgG |
| Jak2 (D2E12) XP® Rabbit mAb 3230 | 20 µl |
|
H M R | 125 | Rabbit IgG |
| Jak3 (D1H3) Rabbit mAb 8827 | 20 µl |
|
H | 115 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Usage Information
Product Description
The Jak Isoform Antibody Sampler Kit provides an economical means to examine several Jak isoforms. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.
Specificity / Sensitivity
Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.
Source / Purification
Each isoform-specific antibody is produced by immunizing animals with a synthetic peptide that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
Data Sheets & Documentation
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791. U.S. Patent No. 5,675,063.
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