Name: Jak Isoform Sampler Kit
Brand: Cell Signaling Technology

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344, in the presence of control peptide (left) or Jak1 Blocking Peptide (right).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP^® Rabbit mAb.

Western blot analysis of extracts from various cell lines using Jak3 (D1H3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP^® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak1 (6G4) Rabbit mAb.

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP^® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP^® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.

Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines using Jak1 (6G4) Rabbit mAb.

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP^® Rabbit mAb.

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To Purchase # 9945T

Product #	Size	Price
9945T	1 Kit (3 x 20 µl)	$ 253

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Jak1 (6G4) Rabbit mAb 3344	20 µl	WB IHC	H M R	130	Rabbit IgG
Jak2 (D2E12) XP^® Rabbit mAb 3230	20 µl	WB IP IHC	H M R	125	Rabbit IgG
Jak3 (D1H3) Rabbit mAb 8827	20 µl	WB IP	H	115	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB			Goat

Kit Usage Information

Protocols

3230: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
3344: Western Blotting, Immunohistochemistry (Paraffin)
7074: Western Blotting
8827: Western Blotting, Immunoprecipitation (Magnetic)

Product Description

The Jak Isoform Antibody Sampler Kit provides an economical means to examine several Jak isoforms. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.

Source / Purification

Each isoform-specific antibody is produced by immunizing animals with a synthetic peptide that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Jak Isoform Sampler Kit #9945