Render Target: STATIC
Render Timestamp: 2024-11-01T09:58:55.711Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:58:29.435
Product last modified at: 2024-09-30T08:01:49.069Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

JMJD7 (E8J8Q) Rabbit mAb #85449

Filter:
  • WB

    Supporting Data

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 35, 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    JMJD7 (E8J8Q) Rabbit mAb recognizes endogenous levels of total JMJD7 protein.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro251 of human JMJD7 protein.

    Background

    The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). JMJD7 is a member of the less studied JmjC family of oxygenates that catalyze formation of alcohol products. JMJD7 uniquely catalyzes (3S)-lysyl hydroxylation in members of the Translation Factor GTPase family (4). JMJD7, along with JMJD5, have also been found to function as proteases capable of cleaving histone tails containing methylated arginine (5,6). The JMJD7 gene can fuse with neighboring gene PLA2G4B to drive head and neck squamous cell carcinomas (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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