Render Target: STATIC
Render Timestamp: 2024-12-09T12:25:48.337Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-11-12 23:02:10.531
Product last modified at: 2024-11-27T22:00:09.277Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

JNK2 (56G8) Rabbit mAb #9258

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk Mi
    SENSITIVITY Endogenous
    MW (kDa) 46, 54
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • Mi-Mink 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #15676.

    Protocol

    Specificity / Sensitivity

    JNK2 (56G8) Rabbit mAb detects endogenous levels of total JNK2 protein. This antibody does not recognize JNK1 or JNK3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Mink

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a human JNK2/MBP fusion protein.

    Background

    The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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