Immunohistochemical analysis of paraffin-embedded mouse colon using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated).
Immunohistochemical analysis of paraffin-embedded mouse heart using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated). Heart is negative, as expected.
Immunohistochemical analysis of paraffin-embedded mouse spleen using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|DETECTION REAGENT/SUBSTRATE COMPATIBILITY|
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 283
Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) recognizes endogenous levels of murine Ki-67 protein. It will also detect endogenous levels of human Ki-67 protein; however, Ki-67 (D2H10) Rabbit mAb (IHC Specific) #9027 is recommended for the detection of human Ki-67 protein in paraffin-embedded tissues.Species Reactivity:
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of Ki-67 protein.
Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.