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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Kir4.1 (F3O7C) Rabbit mAb #42918

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 42, 140, 180
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Kir4.1 (F3O7C) Rabbit mAb recognizes endogenous levels of total Kir4.1 protein. This antibody detects a non-specific band at 70 kDa by western blot. Non-specific staining was observed in frozen formaldehyde fixed mouse lung by immunofluorescence.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro339 of mouse Kir4.1 protein.

    Background

    Inwardly rectifying potassium (Kir) channels containing Kir4.1 subunits (Kir4.1 channels) are expressed exclusively in glial cells in the central nervous system (CNS) (1). These channels provide a spatial buffering system for potassium ions, playing an important role in maintaining neuronal excitability (1-3). Astrocytic Kir4.1 channels have a pathophysiological role in epilepsy, Fragile X syndrome, chronic pain, and depression (2,4,5). Furthermore, Kir4.1 channels are expressed in oligodendrocytes. Oligodendric potassium clearance has been found critical for sustained axonal function and integrity (6).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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