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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

KLC1 (D2T2R) Rabbit mAb #48633

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  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 65, 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KLC1 (D2T2R) Rabbit mAb recognizes endogenous levels of total KLC1 protein. This antibody also cross-reacts with an unidentified protein of 25 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly443 of human KLC1 protein.

    Background

    Kinesins are heterotetrameric motor proteins that transport cargo along microtubule tracks toward their plus ends (anterograde direction) in an ATP-dependent manner. Two heavy chains contain the motor activity, while two kinesin light chains act as adaptor proteins that may be required for binding of specific cargo and/or regulation of heavy chain catalytic activity. The amino terminus of kinesin light chain 1 (KLC1) binds to kinesin heavy chains while the KLC1 carboxy-terminal tetratricopeptide repeat (TPR) domain binds cargo (1-3). Phosphorylation of KLC1 at Ser521 by AMPK may regulate insulin granule dynamics (4,5). Research studies identify a KLC1-ALK fusion protein in human lung adenocarcinoma, and this finding may provide insight for future therapeutics (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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