Cat. # | Size | Qty. | Price |
---|---|---|---|
15306S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 42 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu108 of human KLF2 protein.
Krüppel-like factors (KLFs) are a family of zinc finger-containing DNA-binding transcriptional regulators that share homology with the Drosophila melanogaster Krüppel protein that regulates body segmentation. Seventeen mammalian KLFs have been identified so far and they regulate diverse biological processes, including development, differentiation, proliferation, growth, survival, and external stress responses. Alterations in their functions have been linked to many human diseases, including metabolic diseases and cancer (1-3). KLF2 is a key regulator in the differentiation and function of immune cells and vascular endothelial cells. KLF2 suppresses NF-κB-mediated gene expression and plays important roles in inflammatory response (4-7).
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