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97888
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit #97888

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Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various tissues using ABCA7 (E7O5A) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using Pyk2 (5E2) Mouse mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 3
Immunoprecipitation of ApoE protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ApoE (E7X2A) Rabbit mAb. Western blot analysis was performed using ApoE (E7X2A) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. 
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various tissues and cell lines using ApoE (E7X2A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 5
Immunohistochemical analysis of paraffin-embedded mouse brain using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 6
Confocal immunofluorescent analysis of fixed frozen mouse retina, labeled with ApoE (E7X2A) Rabbit mAb (left, green) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (right, red) and DAPI #4083 (right, blue).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 7
Confocal immunofluorescent analysis of fixed frozen mouse colon, labeled with ApoE (E7X2A) Rabbit mAb (left, green) and co-labeled with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (right, red) and DAPI #4083 (right, blue).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 8
Confocal immunofluorescent analysis of fixed frozen mouse hypothalamus, labeled with ApoE (E7X2A) Rabbit mAb (left, green) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (right, red) and DAPI #4083 (right, blue).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 9
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus, labeled with ApoE (E7X2A) Rabbit mAb (left, green) and co-labeled with GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (right, red) and DAPI #4083 (right, blue).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 10
Confocal immunofluorescent analysis of SIM-A9 cells (left, positive) and C2C12 cells (right, negative) using ApoE (E7X2A) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 11
Western blot analysis of extracts from various cells lines using MEF2C (D80C1) XP® Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 12
Western blot analysis of extracts from various cells and tissue extracts using BIN1 (E4A1P) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 13
Western blot analysis of extracts from mouse bone marrow derived macrophages (BMDM) cells, untreated (-) or treated with peptide N-glycosidase F (PNGase F; +), and Neuro-2a cells using TREM2 (E6T1P) Rabbit mAb (Amino-terminal Antigen, Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 14
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 15
Western blot analysis of extracts from mouse bone marrow derived macrophages (BMDM), untreated (-) or treated with peptide N-glycosidase F (PNGase F; +), and Neuro-2a cells using TREM2 (E7P8J) Rabbit mAb (Carboxy-terminal Antigen, Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 16
Western blot analysis of extracts from various tissues using SORL1 (D8D4G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 17
Western blot analysis of extracts from LoVo, HT-29 and mIMCD-3 cell lines using EphA1 (D6V7I) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 18
Western blot analysis of extracts from various cell lines using ABCA7 (E7O5A) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded mouse kidney using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 20
Confocal immunofluorescent analysis of C2C12 cells, undifferentiated (left) or differentiated for 3 days (right), using MEF2C (D80C1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 21
Immunoprecipitation of BIN1 protein from SK-OV-3 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BIN1 (E4A1P) Rabbit mAb. Western blot analysis was performed using BIN1 (E4A1P) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 22
Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), or cerebellum (right) using SORL1 (D8D4G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 23
Immunoprecipitation of ABCA7 protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ABCA7 (E7O5A) Rabbit mAb. Western blot analysis was performed using ABCA7 Antibody (Mouse Specific) #31954. Anti-rabbit, HRP-linked Antibody #7074 was used as a secondary antibody.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded mouse spleen using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded mouse testis using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded mouse thymus using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded mouse small intestine using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded liver (left) or brain (right) from C57BL/6NTac (wt/wt, model #B6-F, top) and APOE4 knock-in (hu/hu, model #1549-F, bottom) mice using ApoE (E7X2A) Rabbit mAb. Mice from Taconic Biosciences, Inc. Note lack of reactivity in tissues with human APOE4 replacing the mouse gene.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded mouse liver using ApoE (E7X2A) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®  Isotype Control #3900 (right).
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded SIM-A9 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using ApoE (E7X2A) Rabbit mAb.
Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit: Image 33
Western blot analysis of extracts from HepG2 cells (lane 1), 293T mock transfected (lane 2) or transiently transfected with a construct expressing ApoE4 (lane 3), whole liver extracts from wild type C57BL/6NTac model #B6-F mice (lane 4), or ApoE4 knock-in (model #1549-F) (lane 5), whole brain extracts from wild type C57BL/6NTac mice (lane 6), or ApoE4 knock-in (lane 7) using ApoE (E7X2A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Mice from Taconic Biosciences, Inc.
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To Purchase # 97888
Cat. # Size Qty. Price
97888T		 1 Kit  (9 x 20 microliters)
$ 708

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
ABCA7 (E7O5A) Rabbit mAb 32942 20 µl
  • WB
  • IP
 M R 235 Rabbit IgG
SORL1 (D8D4G) Rabbit mAb 79322 20 µl
  • WB
  • IF
 H M 250 Rabbit IgG
BIN1 (E4A1P) Rabbit mAb 51844 20 µl
  • WB
  • IP
 H M 45-80 Rabbit IgG
EphA1 (D6V7I) Rabbit mAb 90673 20 µl
  • WB
 H M 130 Rabbit IgG
MEF2C (D80C1) XP® Rabbit mAb 5030 20 µl
  • WB
  • IP
  • IF
 H M 50-60 Rabbit IgG
Pyk2 (5E2) Mouse mAb 3480 20 µl
  • WB
  • IP
 H M 116 Mouse IgG2a
TREM2 (E6T1P) Rabbit mAb (Amino-terminal Antigen, Mouse Specific) 61788 20 µl
  • WB
 M 28 Rabbit IgG
TREM2 (E7P8J) Rabbit mAb (Carboxy-terminal Antigen, Mouse Specific) 76765 20 µl
  • WB
 M 11, 28 Rabbit IgG
ApoE (E7X2A) Rabbit mAb 49285 20 µl
  • WB
  • IP
  • IHC
  • IF
 M 35 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 

Product Description

The Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit provides an economical means of detecting proteins identified as risk factors for late-onset Alzheimer’s Disease (LOAD) by western blot. This kit includes enough antibodies to perform at least two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Late-Onset Alzheimer's Disease Risk Gene (Mouse Model) Antibody Sampler Kit detects endogenous levels of its target protein. ApoE (E7X2A) Rabbit mAb recognizes endogenous levels of total mouse ApoE protein. Non-specific staining was observed in mouse pancreatic islets by immunohistochemistry and immunofluorescence. Pyk2 (5E2) Mouse mAb detects endogenous levels of total Pyk2 protein. It does not cross-react with other related proteins. BIN1 (E4A1P) Rabbit mAb recognizes endogenous levels of total BIN1 protein. The antibody recognizes multiple BIN1 isoforms. TREM2 (E6T1P) Rabbit mAb (Amino-terminal Antigen, Mouse Specific) recognizes endogenous levels of total TREM2 protein. A non-specific band of unknown origin is observed migrating at ~75 kDa. TREM2 (E7P8J) Rabbit mAb (Carboxy-terminal antigen, Mouse Specific) recognizes endogenous levels of total mouse TREM2 protein, both the full-length and the carboxy-terminal membrane fragment generated by proteolytic processing. A non-specific band of unknown origin is observed migrating at ~80 kDa.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Asp26 of mouse ApoE protein, Val266 of human BIN1 protein, Glu267 of human SORL1 protein, Met182 of human MEF2 protein, Gly215 of mouse TREM2 protein, residues near the amino-terminus of mouse TREM2 protein, and a recombinant protein fragment specific to the extracellular domain of human EphA1 protein. Pyk2 (5E2) Mouse mAb is produced by immunizing animals with a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2 protein.

Background

Alzheimer's Disease (AD) is the leading cause of dementia worldwide. Clinically, it is characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles, which result in neuronal dysfunction and cell death (1). Genome-wide association studies (GWAS) have identified a cohort of risk genes associated with late-onset AD (LOAD), including, but not limited to, APOE, BIN1, SORL1, TREM2, EphA1, MEF2C, ABCA7, and PTK2B (2).
 
APOE has three allele variants; ApoE2, ApoE3, and ApoE4; with ApoE4 associated with an increased risk of AD. Evidence suggests that this risk occurs through promotion of amyloid-beta plaque aggregation (1). ApoE4 is also associated with impaired microglial response, lipid transport, synaptic integrity and plasticity, glucose metabolism, and cerebrovascular integrity (3). Mutations in BIN1, primarily involved in endocytosis and maintaining cytoskeletal integrity in the brain, are suggested to play a role in the aggravation of tau pathology (4,5). Increased levels of BIN1 have been seen in AD postmortem brain tissue (5). SORL1 expression is decreased in the brain of AD patients (6). Studies have demonstrated a role for SORL1 as a neuronal sorting receptor that binds amyloid precursor protein (APP) and regulates its trafficking and proteolytic processing, thus regulating β-amyloid (Aβ) peptide production (7). The triggering receptor expressed on myeloid cells 2 (TREM2) is an innate immune receptor that is expressed on the cell surface of microglia, macrophages, osteoclasts, and immature dendritic cells (8). Research studies using AD mouse models indicate that deficiency and haploinsufficiency of TREM2 can lead to increased Aβ accumulation due to dysfunctional microglia response (9). EphA1 is a member of the ephrin family of receptor tyrosine kinases responsible for regulating cell morphology and motility (10). In the central nervous system (CNS), EphA1 plays a role in synaptic plasticity and axon guidance (11). EphA1 is involved in inflammatory signaling pathways (12), which may mean it plays a role in regulation of neuroinflammatory processes in AD (13). ATP-binding cassette sub-family A member 7 (ABCA7) functions to regulate phospholipid and cholesterol homeostasis in the CNS (14,15). ABCA7 dysfunction may contribute directly to AD pathogenesis by accelerating Aβ production and/or altering microglia-dependent phagocytosis of Aβ (16-18). MEF2C is a member of the myocyte enhancer factor 2 (MEF2) family of transcription factors shown to play a role in learning and memory formation through regulation of synaptic plasticity (19). Studies have shown that MEF2C may play a role in age-related microglial activation through IFN-I associated MEF2C deregulation (20,21). MEF2C may also act as a modulator for APP proteolytic processing of Aβ (22,23). Protein tyrosine kinase, Pyk2, encoded by the PTK2B gene, is a non-receptor tyrosine kinase highly expressed in neurons with implications in synaptic plasticity (24,25). In mouse models, knockout of Pyk2 impairs hippocampal-dependent memory and long-term potentiation (24). Overexpression of Pyk2 has been shown to protect neurons against Aβ42-induced synaptotoxicity (26). Pyk2 may also act as a kinase for tau phosphorylation and has been implicated as a modulator of tau toxicity (27,28).
  1. Selkoe, D.J. (2001) Physiol Rev 81, 741-66.
  2. Zhang, Q. et al. (2020) Nat Commun 11, 4799.
  3. Yamazaki, Y. et al. (2019) Nat Rev Neurol 15, 501-518.
  4. Franzmeier, N. et al. (2019) Nat Commun 10, 1766.
  5. Chapuis, J. et al. (2013) Mol Psychiatry 18, 1225-34.
  6. Scherzer, C.R. et al. (2004) Arch Neurol 61, 1200-5.
  7. Andersen, O.M. et al. (2005) Proc Natl Acad Sci U S A 102, 13461-6.
  8. Colonna, M. (2003) Nat Rev Immunol 3, 445-53.
  9. Wang, Y. et al. (2015) Cell 160, 1061-71.
  10. Yamazaki, T. et al. (2009) J Cell Sci 122, 243-55.
  11. Lai, K.O. and Ip, N.Y. (2009) Curr Opin Neurobiol 19, 275-83.
  12. Ivanov, A.I. and Romanovsky, A.A. (2006) IUBMB Life 58, 389-94.
  13. Villegas-Llerena, C. et al. (2016) Curr Opin Neurobiol 36, 74-81.
  14. Abe-Dohmae, S. et al. (2004) J Biol Chem 279, 604-11.
  15. Wang, N. et al. (2003) J Biol Chem 278, 42906-12.
  16. Pereira, C.D. et al. (2018) J Alzheimers Dis 61, 463-485.
  17. Fu, Y. et al. (2016) J Alzheimers Dis 54, 569-84.
  18. Aikawa, T. et al. (2018) Brain Sci 8, 27.
  19. Rashid, A.J. et al. (2014) Genes Brain Behav 13, 118-25.
  20. Xue, F. et al. (2021) Neurobiol Dis 152, 105272.
  21. Deczkowska, A. et al. (2017) Nat Commun 8, 717.
  22. Tang, S.S. et al. (2016) Oncotarget 7, 39136-39142.
  23. Camargo, L.M. et al. (2015) PLoS One 10, e0115369.
  24. Giralt, A. et al. (2017) Nat Commun 8, 15592.
  25. Mastrolia, V. et al. (2021) Sci Rep 11, 16357.
  26. Kilinc, D. et al. (2020) Brain Commun 2, fcaa139.
  27. Li, C. and Götz, J. (2018) J Alzheimers Dis 64, 205-221.
  28. Dourlen, P. et al. (2017) Mol Psychiatry 22, 874-883.

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