Cell Signaling Technology Logo - Extra Large

Lefty1 Antibody #9620

Filter:
  • WB

Inquiry Info. # 9620

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 40
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Lefty1 Antibody recognizes transfected levels of total lefty1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro233 of human lefty1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Lefty proteins are members of the TGF-β family of cell signaling molecules that are involved in growth and development (1,2). Named for their role in left-right axis determination and their exclusive expression on the left side of the developing mouse embryo, lefty1 and lefty2 contain a cysteine-knot motif that is characteristic of TGF-β related proteins, but lack an alpha-helix and a cysteine residue critical for ligand dimerization (3). Early in vertebrate embryogenesis, lefty represses TGF-β signaling by inhibiting the phosphorylation of Smad2 following activation of the TGF-β receptor (4). Down-regulated very early upon differentiation (5), lefty proteins act as extracellular antagonists of the signaling pathway for Nodal, a TGF-β ligand critical for left-right patterning and formation of the mesoderm and endoderm (6). Similar to other members of the TGF-β superfamily, lefty proproteins undergo cleavage to release a bioactive protein (7). The biologically active 42 kDa lefty precursor and the 28 kDa polypeptide have been shown to induce MAPK activity (7).

    Alternate Names

    BMP-17; BMP17; Left-right determination factor 1; Left-right determination factor B; LEFTB; lefty b; lefty-1; lefty-b; lefty1; leftyb; LFTY1; Protein lefty-1; Protein lefty-B; stra3

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.