Render Target: STATIC
Render Timestamp: 2024-12-03T10:52:45.863Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-11-18 16:03:18.291
Product last modified at: 2024-11-25T23:30:09.656Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LIF (F6X1X) Rabbit mAb #34848

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LIF (F6X1X) Rabbit mAb recognizes endogenous levels of total LIF protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human LIF protein.

    Background

    Leukemia inhibitory factor (LIF) is a pleiotropic member of the interleukin-6 (IL-6) family of cytokines (1). LIF binds the dimeric receptor LIFRα/gp130 to form a functional trimer (2). The trimeric LIF/LIFR/gp130 complex further interacts with JAK1, making it catalytically competent. The activated JAK1 then initiates a tyrosine phosphorylation cascade that stimulates three distinct signaling pathways: JAK/STAT, MAPK, and PI3K pathways (3). During development, LIF plays important roles in embryonic stem cell pluripotency, blastocyst implantation, and early neural development. It is also crucial for intestinal stem cell and neuronal stem cell self-renewal and regeneration, muscle and kidney tissue regeneration after injury, and immune tolerance (4). LIF's regulatory role in cancer is context-dependent. It plays a tumor suppressive role in leukemia, but is overexpressed and exerts an oncogenic effect in solid tumors (4,5). Targeting LIF for cancer therapy is promising for disease management (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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