LIMP-2/SCARB2 (E2Z5F) Rabbit mAb (BSA and Azide Free) #69463
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 54, 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
LIMP-2/SCARB2 (E2Z5F) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LIMP-2/SCARB2 protein. LIMP-2/SCARB2 is glycosylated at multiple sites, and it migrates at 80 kDa and 54 kDa in its glycosylated and unglycosylated states, respectively.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2/SCARB2 protein.
Background
Lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the SCARB2 gene. As a lysosome-enriched protein, LIMP-2/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson’s disease, is targeted to lysosomes by binding directly to LIMP-2/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson’s disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8).
- Reczek, D. et al. (2007) Cell 131, 770-83.
- Blanz, J. et al. (2015) Traffic 16, 1127-36.
- Riboldi, G.M. and Di Fonzo, A.B. (2019) Cells 8, 364.
- Brown, M.S. et al. (2018) Annu Rev Biochem 87, 783-807.
- Heybrock, S. et al. (2019) Nat Commun 10, 3521.
- Pérez-Roca, L. et al. (2019) Neurosci Lett 706, 164-168.
- Hopfner, F. et al. (2013) Mov Disord 28, 538-40.
- Yamayoshi, S. et al. (2009) Nat Med 15, 798-801.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.