Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded cell pellets, LKB1 positive COS-7 (left) and LKB1 negative A549 (right), using LKB1 (D60C5F10) Rabbit mAb (IHC-Formulated).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated).
|REACTIVITY||H M Mk|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|DETECTION REAGENT/SUBSTRATE COMPATIBILITY|
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 283
LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total LKB1 protein.Species Reactivity:
Human, Mouse, Monkey
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LKB1 protein.
LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).
Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).
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