Render Target: STATIC
Render Timestamp:
3/24/2025, 6:34:56 AM EDT
3/24/2025, 10:34:56 AM UTC
Commit: a4bd4fb8969f29f780e26f33c272e3bb2f998a01
XML generation date: 2025-03-07 13:19:08.022
Product last modified at: 2025-03-08T08:27:45.728Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LOXL2 (E3P7Y) Rabbit mAb (BSA and Azide Free) #72903

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #99680. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #99680

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    LOXL2 (E3P7Y) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LOXL2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LOXL2 protein.

    Background

    Lysyl oxidase-like 2 (LOXL2) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (1). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOXL2 has been associated with progression and metastases of many cancer types, including breast cancer and fibrotic disease, all through active remodeling of the ECM (2-5). Additionally, within the cell, LOXL2 has been shown to play a role in EMT, cell polarity, and transcriptional repression (6-8). LOXL2 has become an attractive target for therapeutic intervention in cancer treating, blocking metastasis, and halting fibrosis (9-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SignalStain is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.