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LPCAT3 (E5G9P) Rabbit mAb #72964

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 36
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LPCAT3 (E5G9P) Rabbit mAb recognizes endogenous levels of total LPCAT3 protein. This antibody may detect nonspecific bands at 90 and 160 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human LPCAT3 protein.

    Background

    LPCAT3 (lysophosphatidylcholine acyltransferase 3), also called MBOAT5, is part of the LPCAT family that plays an important role in lipid metabolism (1,2). Its function is to catalyze the incorporation of fatty acids into phospholipids, lysophosphatidylcholine (lysoPC), lysophosphatidylserine (lysoPS), and lysophosphatidylethanolamine (lysoPE), to produce various fatty acid containing forms of PC, PS, and PE. It shows a preference for lysoPC and incorporation of linoleoyl and arachidonoyl chains as fatty acid donors (3,4). LPCAT3 is localized to the endoplasmic reticulum (ER) and is primarily expressed in metabolic tissues, including the liver, pancreas, and adipose tissue (2). It is a direct transcriptional target of LXR, PPARα, and PPARγ, which are lipid activated nuclear receptors that play important roles in regulating lipid homeostasis (2,5-7). Elevated expression of LPCAT3 is associated with the iron-dependent cell death process of ferroptosis that is characterized by an increase in lipid peroxidation (8). LPCAT3 inhibitors have shown a partial protection from ferroptosis (9). Dysregulation of LPCAT3 can have an important role in the development of diseases, including atherosclerosis, diabetes, intestinal tumors, and nonalcoholic steatohepatitis (NASH) (10,11). LPCAT3 is significantly upregulated in many cancers and is associated with prognosis (12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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